Ion-Pair Complex of Trimethoprim with Alizarin Yellow and its Determination and Extraction by LLE and DLLME

For the analysis of trimethoprim medication in its pure form and pharmaceutical formulations, precise accurate microextraction techniques with UV-Vis measurement have been developed confirmed. In this study, LLE DLLME procedures were utilized for separation, pre-concentration, evaluation preparations by spectroscopy at 390 nm. The method was validated applied to formulations. Several experimental factors containing type dispersive extraction solvents their volumes, pH, temperature, centrifuging time carried out. Under ideal conditions, linear range 2–45 1-10 mg/L, a coefficient determination (R2) 0.9961 0.9992 DLLME, respectively. limit detection (LOD) 1.52 0.21 mg/L. Recovery target analyte formulations 99.3%-104.9%.